APEX1 Antibody

• Background:

  Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.

• NCBI Gene ID:

  328

• Swiss Prot:

  P27695

• Accession Number:

  P27695

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  36 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  APEX1

• NCBI Official Name:

  DNA- (apurinic or apyrimidinic site) lyase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Vascotto, C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009)

• Background Reference 02:

  Li, W.Q., Carcinogenesis 30 (3), 500-505 (2009)

• Background Reference 03:

  Lo, Y.L., Cancer Epidemiol. Biomarkers Prev. 18 (1), 223-229 (2009)

• Background Reference 04:

  Bhakat, K.K., EMBO J. 22 (23), 6299-6309 (2003)

• Other Product Names:

  DNA- (apurinic or apyrimidinic site) lyase, 31--, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA- (apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1

• Tested Applications:

  WB, IF

• Protein ID:

  113984

• Physical Properties:

  Liquid