MTHFD1 Antibody

• Background:

  MTHFD1 is a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

• NCBI Gene ID:

  4522

• Swiss Prot:

  P11586

• Accession Number:

  P11586

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MTHFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MTHFD1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  102 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MTHFD1

• NCBI Official Name:

  C-1-tetrahydrofolate synthase, cytoplasmic

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ivanov, A., Am Diet Assoc 109 (2), 313-318 (2009)

• Background Reference 02:

  Schmidt A., Biochemistry 39:6325-6335 (2000)

• Background Reference 03:

  Hol F.A., Clin. Genet. 53:119-125 (1998)

• Other Product Names:

  C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, Formyltetrahydrofolate synthetase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, MTHFD1, MTHFC, MTHFD

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  115206

• Physical Properties:

  Liquid