MTHFD2 Antibody

• Background :

  MTHFD2 is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD.This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

• NCBI Gene ID :

  10797

• Swiss Prot :

  P13995

• Accession Number :

  NP_006627

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTHFD2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Immunology

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MTHFD2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  35 kDa, 27 kDa

• Applications Notes :

  MTHFD2 antibody can be used for detection of MTHFD2 by ELISA at 1:312500. MTHFD2 antibody can be used for detection of MTHFD2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  MTHFD2

• NCBI Official Name :

  Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Kimura, K., (2006) Genome Res. 16 (1), 55-65.

• Other Product Names :

  MTHFD2, NMDMC

• Tested Applications :

  ELISA, WB

• Protein ID :

  94721354

• Physical Properties :

  Liquid