MTHFD2 Antibody

• Background:

  This protein is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

• NCBI Gene ID:

  10797

• Swiss Prot:

  P13995

• Accession Number:

  NP_006627

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTHFD2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MTHFD2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Applications Notes:

  MTHFD2 antibody can be used for detection of MTHFD2 by ELISA at 1:312500. MTHFD2 antibody can be used for detection of MTHFD2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MTHFD2

• NCBI Official Name:

  Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hillier, L.W., (2005) Nature 434 (7034), 724-731.

• Other Product Names:

  MTHFD2, NMDMC

• Tested Applications:

  ELISA, WB

• Protein ID:

  94721354

• Physical Properties:

  Liquid