EIF5 Antibody

• Background:

  Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .

• NCBI Gene ID:

  1983

• Swiss Prot:

  P55010

• Accession Number:

  P55010

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EIF5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 267-298 amino acids from the Central region of human EIF5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  49 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  EIF5

• NCBI Official Name:

  Eukaryotic translation initiation factor 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Si K., et al.J. Biol. Chem. 271:16934-16938 (1996) .

• Background Reference 02:

  Wiemann S., et al.Genome Res. 11:422-435 (2001) .

• Background Reference 03:

  Ota T., et al.Nat. Genet. 36:40-45 (2004) .

• Background Reference 04:

  Bechtel S., et al.BMC Genomics 8:399-399 (2007) .

• Other Product Names:

  Eukaryotic translation initiation factor 5, eIF-5, EIF5

• Tested Applications:

  WB

• Protein ID:

  27735202

• Physical Properties:

  Liquid