EIF5B Antibody

• Background:

  Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

• NCBI Gene ID:

  9669

• Swiss Prot:

  O60841

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  DU145

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 179kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  EIF5B

• Positive Control 2:

  HeLa

• Positive Control 3:

  Jurkat

• Positive Control 4:

  LO2

• NCBI Official Name:

  Eukaryotic translation initiation factor 5B

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  IF2, eukaryotic translation initiation factor 5B, eIF-5B, translation initiation factor IF-2, translation initiation factor IF2

• Tested Applications:

  WB

• Physical Properties:

  Liquid