NUP85 Antibody

• Background:

  Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade.

• NCBI Gene ID:

  79902

• Swiss Prot:

  Q9BW27

• Accession Number:

  Q9BW27

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NUP85 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-195 amino acids from human NUP85.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  75 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  NUP85

• NCBI Official Name:

  Nuclear pore complex protein Nup85

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cronshaw J.M., et al.J. Cell Biol. 158:915-927 (2002) .

• Background Reference 02:

  Terashima Y., et al.Nat. Immunol. 6:827-835 (2005) .

• Background Reference 03:

  Ota T., et al.Nat. Genet. 36:40-45 (2004) .

• Background Reference 04:

  Bechtel S., et al.BMC Genomics 8:399-399 (2007) .

• Other Product Names:

  Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1

• Tested Applications:

  WB

• Protein ID:

  74733394

• Physical Properties:

  Liquid