RNPS1 Antibody

• Background:

  Part of pre- and post-splicing multiprotein mRNP complexes. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes) ; specifically inhibits formation of proapoptotic isoforms such as Bcl-X (S) ; the activity is different from the established EJC assembly and function. Participates in mRNA 3'-end cleavage. Involved in UPF2- dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions.

• NCBI Gene ID:

  10921

• Swiss Prot:

  Q15287

• Accession Number:

  Q15287

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RNPS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-44 amino acids from the RNPS1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:500-1:1000

• Symbol:

  RNPS1

• NCBI Official Name:

  RNA-binding protein with serine-rich domain 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Badolato J., et al.Gene 166:323-327 (1995) .

• Background Reference 02:

  Loyer P., et al.J. Cell Sci. 111:1495-1506 (1998) .

• Background Reference 03:

  Harada K., et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.

• Background Reference 04:

  Ota T., et al.Nat. Genet. 36:40-45 (2004) .

• Other Product Names:

  RNA-binding protein with serine-rich domain 1, SR-related protein LDC2, RNPS1, LDC2

• Tested Applications:

  WB

• Protein ID:

  74754492

• Physical Properties:

  Liquid