MBNL1 Antibody

• Background:

  Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU (U/G) Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

• NCBI Gene ID:

  4154

• Swiss Prot:

  Q9NR56

• Accession Number:

  Q9NR56

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MBNL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the C-terminal region of human MBNL1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  42 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MBNL1

• NCBI Official Name:

  Muscleblind-like protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sen, S., et al. J. Biol. Chem. 285 (33) :25426-25437 (2010)

• Background Reference 02:

  Arambula, J.F., et al. Proc. Natl. Acad. Sci. U.S.A. 106 (38) :16068-16073 (2009)

• Background Reference 03:

  Cardani, R., et al. Neuromuscul. Disord. 19 (5) :335-343 (2009)

• Background Reference 04:

  Holt, I., et al. Am. J. Pathol. 174 (1) :216-227 (2009)

• Other Product Names:

  Muscleblind-like protein 1, Triplet-expansion RNA-binding protein, MBNL1, EXP, KIAA0428, MBNL

• Tested Applications:

  WB

• Protein ID:

  17369313

• Physical Properties:

  Liquid