POLK Antibody

• Background:

  External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]) .

• NCBI Gene ID:

  51426

• Swiss Prot:

  Q9UBT6

• Accession Number:

  Q9UBT6

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  99 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POLK

• NCBI Official Name:

  DNA polymerase kappa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :

• Background Reference 03:

  Katafuchi, A., et al. Nucleic Acids Res. 38 (3) :859-867 (2010)

• Background Reference 04:

  Fukuda, H., et al. J. Biol. Chem. 284 (38) :25585-25592 (2009)

• Other Product Names:

  DNA polymerase kappa, DINB protein, DINP, POLK, DINB1

• Tested Applications:

  WB

• Protein ID:

  59798438

• Physical Properties:

  Liquid