POLG Antibody

• Background:

  Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) . Two transcript variants encoding the same protein have been found for this gene.

• NCBI Gene ID:

  5428

• Swiss Prot:

  P54098

• Accession Number:

  P54098

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  140 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  POLG

• NCBI Official Name:

  DNA polymerase subunit gamma-1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tong, Z.B., et al. Fertil. Steril. 94 (7) :2932-2934 (2010)

• Background Reference 02:

  Stewart, J.D., et al. Hepatology 52 (5) :1791-1796 (2010)

• Background Reference 03:

  Batabyal, D., et al. J. Biol. Chem. 285 (44) :34191-34201 (2010)

• Background Reference 04:

  Wang, W., et al. Nucleic Acids Res. (2010) In press :

• Other Product Names:

  DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA

• Tested Applications:

  WB, IHC-P, IF, Flow

• Protein ID:

  1706507

• Physical Properties:

  Liquid