Mouse Xaf1 Antibody

• Background:

  Xaf1 seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity) .

• NCBI Gene ID:

  327959

• Swiss Prot:

  Q5NBU8

• Accession Number:

  Q5NBU8

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Xaf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of mouse Xaf1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  31 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Xaf1

• NCBI Official Name:

  XIAP-associated factor 1

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Bai, Y., et al. J. Biol. Chem. 283 (11) :6832-6842 (2008)

• Background Reference 02:

  Qiao, L., et al. Tumour Biol. 29 (2) :122-129 (2008)

• Background Reference 03:

  Wang, X., et al. Neurobiol. Dis. 16 (1) :179-189 (2004)

• Background Reference 04:

  Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100 (24) :14109-14114 (2003)

• Other Product Names:

  XIAP-associated factor 1, BIRC4-binding protein, Xaf1, Birc4bp, Xiapaf1

• Tested Applications:

  WB

• Physical Properties:

  Liquid