IsocitRe dehydrogenase Antibody

• Background:

  Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+) -dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD (+) -dependent isocitrate dehydrogenase.

• NCBI Gene ID:

  3419

• Swiss Prot:

  P50213

• Accession Number:

  P50213

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Isocitrate dehydrogenase (IDH3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human Isocitrate dehydrogenase (IDH3) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  IDH3A

• NCBI Official Name:

  Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Soundar, S., et al., J. Biol. Chem. 278 (52) :52146-52153 (2003) .

• Background Reference 02:

  Weiss, C., et al., Biochemistry 39 (7) :1807-1816 (2000) .

• Background Reference 03:

  Kim, Y.O., et al., J. Biol. Chem. 274 (52) :36866-36875 (1999) .

• Background Reference 04:

  Huh, T.L., et al., Genomics 32 (2) :295-296 (1996) .

• Other Product Names:

  Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD (+) -specific ICDH subunit alpha, IDH3A

• Tested Applications:

  WB

• Protein ID:

  1708399

• Physical Properties:

  Liquid