Elongin A Antibody

• Background:

  Elongin A is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

• NCBI Gene ID:

  6924

• Swiss Prot:

  Q14241

• Accession Number:

  Q14241

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This Elongin A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the N-terminal region of human Elongin A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  90 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TCEB3

• NCBI Official Name:

  Transcription elongation factor B polypeptide 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101 (33) :12130-12135 (2004) .

• Background Reference 02:

  Tamura, K., et al., Biochem. Biophys. Res. Commun. 309 (1) :189-195 (2003) .

• Background Reference 03:

  Kile, B.T., et al., Trends Biochem. Sci. 27 (5) :235-241 (2002) .

• Background Reference 04:

  Yamazaki, K., et al., J. Biol. Chem. 277 (29) :26444-26451 (2002) .

• Other Product Names:

  Transcription elongation factor B polypeptide 3, Elongin 110 kDa subunit, Elongin-A, EloA, RNA polymerase II transcription factor SIII subunit A1, SIII p110, TCEB3

• Tested Applications:

  WB

• Protein ID:

  294862430

• Physical Properties:

  Liquid