PODXL Antibody

• Background:

  This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

• NCBI Gene ID:

  5420

• Swiss Prot:

  O00592

• Accession Number:

  O00592

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PODXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  59 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PODXL

• NCBI Official Name:

  Podocalyxin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398 (3) :372-376 (2010)

• Background Reference 02:

  Hsu, Y.H., et al. Am. J. Pathol. 176 (6) :3050-3061 (2010)

• Background Reference 03:

  Johnatty, S.E., et al. PLoS Genet. (7), E1001016 (2010) :

• Background Reference 04:

  Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009) :

• Other Product Names:

  Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1

• Tested Applications:

  WB

• Physical Properties:

  Liquid