CHST5 Antibody

• Background:

  The carbohydrates of glycoconjugates are highly diverse structures with variation in monosaccharide composition, glycosidic linkage positions, and branching of chains. Further diversity is added by the covalent addition of sulfate moieties to particular hydroxyl groups and amino groups of saccharides. The sulfate modifications of glycoproteins can be extensive in amount and frequently occur at high density. They can have a profound effect on the physiochemical properties of the glycoconjugates, at least in part through the addition of negative charge. Carbohydrate sulfation plays a critical role in many biologic processes. CHST5 belongs to the GST family of sulfotransferases, which also includes CHST1 (MIM 603797), CHST2 (MIM 603798), CHST3 (MIM 603799), and LSST. These enzymes are 6-O-sulfotransferases, which add sulfate to C6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) (Lee et al., 1999 [PubMed 10491328]) .

• NCBI Gene ID:

  23563

• Swiss Prot:

  Q9GZS9

• Accession Number:

  Q9GZS9

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CHST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-302 amino acids from the C-terminal region of human CHST5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  46 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CHST5

• NCBI Official Name:

  Carbohydrate sulfotransferase 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Liu, C.Y., et al. Carcinogenesis 31 (7) :1259-1263 (2010)

• Background Reference 02:

  Dastani, Z., et al. Eur. J. Hum. Genet. 18 (3) :342-347 (2010)

• Background Reference 03:

  Saito, A., et al. J. Hum. Genet. 54 (6) :317-323 (2009)

• Background Reference 04:

  Kitayama, K., et al. J. Biol. Chem. 282 (41) :30085-30096 (2007)

• Other Product Names:

  Carbohydrate sulfotransferase 5, 282-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha, GST4-alpha, Intestinal N-acetylglucosamine-6-O-sulfotransferase, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, hIGn6ST, N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-3, Gn6st-3, CHST5

• Tested Applications:

  WB

• Protein ID:

  239938912

• Physical Properties:

  Liquid