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CHST1 Antibody

CAT: 0223-30-071Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-30-071Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CHST1 catalyzes the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. It has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. It may function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. It participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
NCBI Gene ID
8534
Swiss Prot
O43916
Accession Number
NP_003645
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHST1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Signal Transduction
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHST1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
47 kDa
Applications Notes
CHST1 antibody can be used for detection of CHST1 by ELISA at 1:1562500. CHST1 antibody can be used for detection of CHST1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
CHST1
NCBI Official Name
Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
NCBI Organism
Homo sapiens
Background Reference 01
Yamada, T., Biochem. J. 384 (PT 3), 567-575 (2004) .
Other Product Names
CHST1, C6ST, KS6ST, KSGal6ST, KSST, GST-1
Tested Applications
ELISA, WB, IHC
Protein ID
4502841
Physical Properties
Liquid

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