API5 Antibody

• Background:

  Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

• NCBI Gene ID:

  8539

• Swiss Prot:

  Q9BZZ5

• Accession Number:

  Q9BZZ5

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-510 amino acids from the C-terminal region of human API5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  59 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  API5

• NCBI Official Name:

  Apoptosis inhibitor 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :

• Background Reference 02:

  Ren, K., et al. Pathol. Oncol. Res. 16 (2) :229-237 (2010)

• Background Reference 03:

  Need, A.C., et al. Hum. Mol. Genet. 18 (23) :4650-4661 (2009)

• Background Reference 04:

  Rigou, P., et al. EMBO J. 28 (11) :1576-1588 (2009)

• Other Product Names:

  Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5

• Tested Applications:

  WB

• Protein ID:

  353526346

• Physical Properties:

  Liquid