HECTD3 Antibody

• Background:

  E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity) .

• NCBI Gene ID:

  79654

• Swiss Prot:

  Q5T447

• Accession Number:

  Q5T447

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HECTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 740-769 amino acids from the C-terminal region of human HECTD3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  97 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  HECTD3

• NCBI Official Name:

  E3 ubiquitin-protein ligase HECTD3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yu, J., et al. Biochem. Biophys. Res. Commun. 367 (4) :805-812 (2008)

• Background Reference 02:

  Gevaert, K., et al. Nat. Biotechnol. 21 (5) :566-569 (2003)

• Other Product Names:

  E3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3

• Tested Applications:

  WB

• Protein ID:

  74744877

• Physical Properties:

  Liquid