PRIM2 Antibody

• Background:

  The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the large, 58 kDa primase subunit. [provided by RefSeq].

• NCBI Gene ID:

  5558

• Swiss Prot:

  P49643

• Accession Number:

  P49643

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-345 amino acids from the Central region of human PRIM2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  59 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PRIM2

• NCBI Official Name:

  DNA primase large subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Vaithiyalingam, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (31) :13684-13689 (2010)

• Background Reference 02:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 03:

  Weiner, B.E., et al. J. Biol. Chem. 282 (46) :33444-33451 (2007)

• Background Reference 04:

  Rush, J., et al. Nat. Biotechnol. 23 (1) :94-101 (2005)

• Other Product Names:

  DNA primase large subunit, 277-, DNA primase 58 kDa subunit, p58, PRIM2, PRIM2A

• Tested Applications:

  WB

• Protein ID:

  51338777

• Physical Properties:

  Liquid