PRIM1 Antibody

CAT: 0223-30-134Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-30-134Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
NCBI Gene ID
5557
Swiss Prot
P49642
Accession Number
NP_000937
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRIM1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1204 - K562 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRIM1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
50 kDa
Applications Notes
PRIM1 antibody can be used for detection of PRIM1 by ELISA at 1:1562500. PRIM1 antibody can be used for detection of PRIM1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PRIM1
NCBI Official Name
Primase, DNA, polypeptide 1 (49kDa)
NCBI Organism
Homo sapiens
Background Reference 01
Arezi, B., (1999) Biochemistry 38 (39), 12899-12907.
Other Product Names
PRIM1, MGC12308, p49
Tested Applications
ELISA, WB
Protein ID
4506051
Physical Properties
Liquid

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