PRIM1 Antibody

• Background:

  The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

• NCBI Gene ID:

  5557

• Swiss Prot:

  P49642

• Accession Number:

  NP_000937

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRIM1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRIM1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  50 kDa

• Applications Notes:

  PRIM1 antibody can be used for detection of PRIM1 by ELISA at 1:1562500. PRIM1 antibody can be used for detection of PRIM1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PRIM1

• NCBI Official Name:

  Primase, DNA, polypeptide 1 (49kDa)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Arezi, B., (1999) Biochemistry 38 (39), 12899-12907.

• Other Product Names:

  PRIM1, MGC12308, p49

• Tested Applications:

  ELISA, WB

• Protein ID:

  4506051

• Physical Properties:

  Liquid