POLR2D Antibody

• Background:

  This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

• NCBI Gene ID:

  5433

• Swiss Prot:

  O15514

• Accession Number:

  O15514

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the Central region of human POLR2D.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  16 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POLR2D

• NCBI Official Name:

  DNA-directed RNA polymerase II subunit RPB4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

• Background Reference 02:

  Meka, H., et al. Nucleic Acids Res. 33 (19) :6435-6444 (2005)

• Background Reference 03:

  Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100 (22) :12666-12671 (2003)

• Background Reference 04:

  Kaehlcke, K., et al. Mol. Cell 12 (1) :167-176 (2003)

• Other Product Names:

  DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D

• Tested Applications:

  WB

• Physical Properties:

  Liquid