POLR2B Antibody

• Background:

  This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].

• NCBI Gene ID:

  5431

• Swiss Prot:

  P30876

• Accession Number:

  P30876

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  134 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POLR2B

• NCBI Official Name:

  DNA-directed RNA polymerase II subunit RPB2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

• Background Reference 03:

  Tu, S., et al. Nat. Struct. Mol. Biol. 15 (4) :419-421 (2008)

• Background Reference 04:

  Lee, J.H., et al. Mol. Cell. Biol. 28 (2) :609-618 (2008)

• Other Product Names:

  DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B

• Tested Applications:

  WB

• Protein ID:

  401012

• Physical Properties:

  Liquid