CDC42EP2 Antibody

• Background:

  CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. Coexpression of this protein with dominant negative mutant CDC42 protein in fibroblast was found to induce pseudopodia formation, which suggested a role of this protein in actin filament assembly and cell shape control.

• NCBI Gene ID:

  10435

• Swiss Prot:

  O14613

• Accession Number:

  O14613

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This CDC42EP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human CDC42EP2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  22 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CDC42EP2

• NCBI Official Name:

  Cdc42 effector protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xue, Y., et al. Int. J. Cancer 118 (12) :2965-2972 (2006)

• Background Reference 02:

  Joberty, G., et al. Nat. Cell Biol. 3 (10) :861-866 (2001)

• Background Reference 03:

  Hirsch, D.S., et al. J. Biol. Chem. 276 (2) :875-883 (2001)

• Background Reference 04:

  Joberty, G., et al. Mol. Cell. Biol. 19 (10) :6585-6597 (1999)

• Other Product Names:

  Cdc42 effector protein 2, Binder of Rho GTPases 1, CDC42EP2, BORG1, CEP2

• Tested Applications:

  WB

• Physical Properties:

  Liquid