PRKAG3 Antibody

• Background:

  The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq].

• NCBI Gene ID:

  53632

• Swiss Prot:

  Q9UGI9

• Accession Number:

  Q9UGI9

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human PRKAG3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  54 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PRKAG3

• NCBI Official Name:

  5'-AMP-activated protein kinase subunit gamma-3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jablonski, K.A., et al. Diabetes 59 (10) :2672-2681 (2010)

• Background Reference 02:

  Jassim, G., et al. Pharmacopsychiatry (2010) In press :

• Background Reference 03:

  Crawford, S.A., et al. Diabetologia 53 (9) :1986-1997 (2010)

• Background Reference 04:

  Ramanathan, L., et al. Protein Expr. Purif. 70 (1) :13-22 (2010)

• Other Product Names:

  5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3

• Tested Applications:

  WB

• Protein ID:

  85681287

• Physical Properties:

  Liquid