Mouse Cdk8 Antibody

• Background :

  Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (By similarity) .

• NCBI Gene ID :

  264064

• Swiss Prot :

  Q8R3L8

• Accession Number :

  Q8R3L8

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This Mouse Cdk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-272 amino acids from the Central region of mouse Cdk8.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Zebrafish

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  53 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  Cdk8

• NCBI Official Name :

  Cyclin-dependent kinase 8

• NCBI Organism :

  Mus musculus

• Background Reference 01 :

  Alarcon, C., et al. Cell 139 (4) :757-769 (2009)

• Background Reference 02 :

  Westerling, T., et al. Mol. Cell. Biol. 27 (17) :6177-6182 (2007)

• Background Reference 03 :

  Jang, M.K., et al. Mol. Cell 19 (4) :523-534 (2005)

• Background Reference 04 :

  Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

• Other Product Names :

  Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8

• Tested Applications :

  WB

• Physical Properties :

  Liquid