Mouse Syk Antibody

• Background:

  Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion. Phosphorylates USP25 and regulates its intracellular levels (By similarity) .

• NCBI Gene ID:

  20963

• Swiss Prot:

  P48025

• Accession Number:

  P48025

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Mouse Syk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of mouse Syk.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  71 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Syk

• NCBI Official Name:

  Tyrosine-protein kinase SYK

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Heizmann, B., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (43) :18563-18568 (2010)

• Background Reference 02:

  Fernandez, P.L., et al. J. Biol. Chem. 285 (43) :32844-32851 (2010)

• Background Reference 03:

  Zou, W., et al. J. Cell. Sci. 123 (PT 17), 2955-2963 (2010) :

• Background Reference 04:

  Falet, H., et al. J. Exp. Med. 207 (9) :1967-1979 (2010)

• Other Product Names:

  Tyrosine-protein kinase SYK, Spleen tyrosine kinase, Syk, ptk72, Sykb

• Tested Applications:

  WB

• Physical Properties:

  Liquid