NME1 Antibody

• Background:

  This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

• NCBI Gene ID:

  4830

• Swiss Prot:

  P15531

• Accession Number:

  P15531

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the Central region of human NME1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  17 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NME1

• NCBI Official Name:

  Nucleoside diphosphate kinase A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Boissan, M., et al. Cancer Res. 70 (19) :7710-7722 (2010)

• Background Reference 02:

  Wang, P.H., et al. Gynecol. Oncol. 119 (1) :70-75 (2010)

• Background Reference 03:

  Conery, A.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (35) :15461-15466 (2010)

• Background Reference 04:

  Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :

• Other Product Names:

  Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23

• Tested Applications:

  WB

• Protein ID:

  127981

• Physical Properties:

  Liquid