DKK4 Antibody

• Background:

  DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

• NCBI Gene ID:

  27121

• Swiss Prot:

  Q9UBT3

• Accession Number:

  Q9UBT3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human DKK4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  25 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  DKK4

• NCBI Official Name:

  Dickkopf-related protein 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Krupnik, V.E., et al., Gene 238 (2) :301-313 (1999) .

• Background Reference 02:

  Yoshida, S., et al., Cytogenet. Cell Genet. 94 (1-2), 88-89 (2001) .

• Other Product Names:

  Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4

• Tested Applications:

  WB, IHC-P

• Protein ID:

  13124092

• Physical Properties:

  Liquid