DKK2 Antibody

• Background:

  The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

• NCBI Gene ID:

  27123

• Swiss Prot:

  Q9UBU2

• Accession Number:

  Q9UBU2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-62 amino acids from the N-terminal region of human DKK2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  28 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  DKK2

• NCBI Official Name:

  Dickkopf-related protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Clark, H.F., et al., Genome Res. 13 (10) :2265-2270 (2003) . Brott, B.K., et al., Mol. Cell. Biol. 22 (17) :6100-6110 (2002) . Krupnik, V.E., et al., Gene 238 (2) :301-313 (1999) .

• Other Product Names:

  Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2

• Tested Applications:

  WB, IHC-P

• Protein ID:

  13124093

• Physical Properties:

  Liquid