PRKAR2A Antibody, KO Validated

• Background:

  CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) .

• NCBI Gene ID:

  5576

• Swiss Prot:

  P13861

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Antibody is Knockout validated.

• Field of Research:

  Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 46kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:1000 - 1:2000

• Symbol:

  PRKAR2A

• Positive Control 2:

  Jurkat

• Positive Control 3:

  SW480

• Positive Control 4:

  LO2

• Positive Control 5:

  Mouse lung

• Positive Control 6:

  Rat skeletal muscle

• NCBI Official Name:

  Protein kinase cAMP-dependent type II regulatory subunit alpha

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, cAMP-dependent protein kinase regulatory subunit RII alpha, protein kinase A, RII-alpha subunit, protein kinase, cAMP-dependent, regulatory subunit type II alpha

• Tested Applications:

  WB

• Physical Properties:

  Liquid