POLR2E Antibody

• Background:

  This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

• NCBI Gene ID:

  5434

• Swiss Prot:

  P19388

• Accession Number:

  P19388

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human POLR2E.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  25 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POLR2E

• NCBI Official Name:

  DNA-directed RNA polymerases I, II, and III subunit RPABC1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

• Background Reference 02:

  Cloutier, P., et al. Methods 48 (4) :381-386 (2009)

• Background Reference 03:

  Michiels, S., et al. Carcinogenesis 30 (5) :763-768 (2009)

• Background Reference 04:

  Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

• Other Product Names:

  DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E

• Tested Applications:

  WB

• Protein ID:

  116242767

• Physical Properties:

  Liquid