RASA1 Antibody

• Background:

  The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

• NCBI Gene ID:

  5921

• Swiss Prot:

  P20936

• Accession Number:

  P20936

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RASA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 966-993 amino acids from the C-terminal region of human RASA1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  116 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RASA1

• NCBI Official Name:

  Ras GTPase-activating protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hemerly, J.P., et al. Eur. J. Endocrinol. 163 (5) :747-755 (2010)

• Background Reference 02:

  Wiemels, J.L., et al. Blood Cells Mol. Dis. 45 (3) :186-191 (2010)

• Background Reference 03:

  Bachas, C., et al. Blood 116 (15) :2752-2758 (2010)

• Background Reference 04:

  Oinuma, I., et al. J. Biol. Chem. 285 (36) :28200-28209 (2010)

• Other Product Names:

  Ras GTPase-activating protein 1, GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP, RASA1, GAP, RASA

• Tested Applications:

  WB

• Protein ID:

  121743

• Physical Properties:

  Liquid