GCAT Antibody

• Background:

  The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

• NCBI Gene ID:

  23464

• Swiss Prot:

  O75600

• Accession Number:

  O75600

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-128 amino acids from the N-terminal region of human GCAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  45 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  GCAT

• NCBI Official Name:

  2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jacquot, C., et al. Anticancer Res. 22 (4) :2229-2235 (2002)

• Background Reference 02:

  Edgar, A.J., et al. Eur. J. Biochem. 267 (6) :1805-1812 (2000)

• Background Reference 03:

  Tressel, T., et al. J. Biol. Chem. 261 (35) :16428-16437 (1986)

• Other Product Names:

  2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

• Tested Applications:

  WB

• Physical Properties:

  Liquid