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POLR1B Antibody

CAT: 0223-56-464Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-56-464Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]) .
NCBI Gene ID
84172
Swiss Prot
Q9H9Y6
Accession Number
Q9H9Y6
Host
Rabbit
Reactivity
Human
Immunogen
This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
128 kDa
Fragment
Rabbit Ig
Symbol
POLR1B
NCBI Official Name
DNA-directed RNA polymerase I subunit RPA2
NCBI Organism
Homo sapiens
Background Reference 01
Wen, L., et al. Biochem. Biophys. Res. Commun. 367 (4) :846-851 (2008)
Background Reference 02
Wu, C., et al. Proteomics 7 (11) :1775-1785 (2007)
Background Reference 03
Johnson, S.S., et al. Mol. Cell 26 (3) :367-379 (2007)
Background Reference 04
Panova, T.B., et al. Mol. Cell. Biol. 26 (16) :5957-5968 (2006)
Other Product Names
DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B
Tested Applications
WB, IHC-P, Flow
Protein ID
92090637
Physical Properties
Liquid

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