POLR2B Antibody

• Background:

  POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

• NCBI Gene ID:

  5431

• Swiss Prot:

  P30876

• Accession Number:

  NP_000929

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POLR2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  134 kDa

• Applications Notes:

  POLR2B antibody can be used for detection of POLR2B by ELISA at 1:312500. POLR2B antibody can be used for detection of POLR2B by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  POLR2B

• NCBI Official Name:

  Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Langelier, M.F., et al., (2005) (er) Nucleic Acids Res. 33 (8), 2629-2639.

• Other Product Names:

  POLR2B, RPB2, POL2RB, hsRPB2, hRPB140

• Tested Applications:

  ELISA, WB

• Protein ID:

  4505941

• Physical Properties:

  Liquid