MED6 Antibody

• Background:

  Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

• NCBI Gene ID:

  10001

• Swiss Prot:

  O75586

• Accession Number:

  O75586

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human MED6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  28 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MED6

• NCBI Official Name:

  Mediator of RNA polymerase II transcription subunit 6

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pavri, R., et al. Mol. Cell 18 (1) :83-96 (2005)

• Background Reference 02:

  Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)

• Background Reference 03:

  Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (8) :2339-2344 (2004)

• Background Reference 04:

  Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

• Other Product Names:

  Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, ARC33, Mediator complex subunit 6, hMed6, Renal carcinoma antigen NY-REN-28, MED6, ARC33

• Tested Applications:

  WB

• Physical Properties:

  Liquid