TAF1 Antibody

• Background:

  Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.

• NCBI Gene ID:

  6872

• Swiss Prot:

  P21675

• Accession Number:

  P21675

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This TAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1863-1893 amino acids from the C-terminal region of human TAF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  213 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TAF1

• NCBI Official Name:

  Transcription initiation factor TFIID subunit 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tavassoli, P., et al. Mol. Endocrinol. 24 (4) :696-708 (2010)

• Background Reference 02:

  Allende-Vega, N., et al. Mol. Cell. Biochem. 316 (1-2), 99-106 (2008) :

• Background Reference 03:

  Centeno, F., et al. Intervirology 51 (2) :137-143 (2008)

• Background Reference 04:

  Olejnik-Schmidt, A.K., et al. Arch. Virol. 153 (5) :983-990 (2008)

• Other Product Names:

  Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, p250, Transcription initiation factor TFIID 250 kDa subunit, TAF (II) 250, TAFII-250, TAFII250, TAF1, BA2R, CCG1, CCGS, TAF2A

• Tested Applications:

  WB

• Protein ID:

  115942

• Physical Properties:

  Liquid