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TAF15 Antibody

CAT: 0223-27-294Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-27-294Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF15 encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.
NCBI Gene ID
8148
Swiss Prot
Q92804
Accession Number
NP_631961
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF15.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF15 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
62 kDa
Applications Notes
TAF15 antibody can be used for detection of TAF15 by ELISA at 1:312500. TAF15 antibody can be used for detection of TAF15 by western blot at 3.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
TAF15
NCBI Official Name
TAF15 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 68kDa
NCBI Organism
Homo sapiens
Background Reference 01
Lee, H.J., et al., (2004) FEBS Lett. 564 (1-2), 188-198.
Other Product Names
TAF15, Npl3, RBP56, TAF2N, TAFII68
Tested Applications
ELISA, WB
Protein ID
21327701
Physical Properties
Liquid

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