RAD17 Antibody

• Background:

  The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

• NCBI Gene ID:

  5884

• Swiss Prot:

  O75943

• Accession Number:

  O75943

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  77 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  RAD17

• NCBI Official Name:

  Cell cycle checkpoint protein RAD17

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang, L., et al. EMBO J. 29 (10) :1726-1737 (2010)

• Background Reference 02:

  Vega, A., et al. Gynecol. Oncol. 112 (1) :210-214 (2009)

• Background Reference 03:

  Beretta, G.L., et al. Cancer Lett. 266 (2) :194-202 (2008)

• Background Reference 04:

  Zhao, M., et al. Head Neck 30 (1) :35-42 (2008)

• Other Product Names:

  Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L

• Tested Applications:

  WB, Flow

• Physical Properties:

  Liquid