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Profilin Antibody

CAT: 0223-36-354Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0223-36-354Size:10 µg
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Background
Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18) .
NCBI Gene ID
5216
Swiss Prot
P07737
Accession Number
4826898
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Pig, Rat
Immunogen
Human full-length profilin.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Innate Immunity, Neuroscience
Purification
Antigen affinity purified.
Concentration
100 ug/ml
Buffer
Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Specificity
Recognizes human, mouse, rat, pig, bovine and marsupial profilin.
Applications Notes
ELISA: (direct or indirect: 1:2,000-1:5,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.
Symbol
PFN1
NCBI Official Name
Profilin 1
NCBI Organism
Homo sapiens
Other Product Names
PFN1
Tested Applications
ICC, IHC, WB
Protein ID
NP_005013
Physical Properties
Liquid

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