Nampt Antibody

• Background:

  Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+) . Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt) . While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

• NCBI Gene ID:

  10135

• Swiss Prot:

  P43490

• Accession Number:

  5031977

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide corresponding to aa 413-431 (V413FKDPVADPNKRSKKGRLS431) of human Nampt (Visfatin/PBEF) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.

• Applications Notes:

  ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections, 10ug/ml) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  NAMPT

• NCBI Official Name:

  Nicotinamide phosphoribosyltransferase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

• Tested Applications:

  ELISA, IHC, WB

• Protein ID:

  NP_005737

• Physical Properties:

  Liquid