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Nampt Antibody

CAT: 0223-36-223Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0223-36-223Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
NCBI Gene ID
10135
Swiss Prot
P43490
Accession Number
5031977
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human Nampt (visfatin/PBEF) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 6 months after receipt when stored at -20˚ C.
Specificity
Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
Applications Notes
ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections (1:500-1:1,000) ) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Symbol
NAMPT
NCBI Official Name
Nicotinamide phosphoribosyltransferase
NCBI Organism
Homo sapiens
Other Product Names
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Tested Applications
ELISA, IHC, WB
Protein ID
NP_005737
Physical Properties
Liquid

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