Nampt Antibody

• Background:

  Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

• NCBI Gene ID:

  10135

• Swiss Prot:

  P43490

• Accession Number:

  5031977

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human Nampt (visfatin/PBEF) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.

• Applications Notes:

  ELISA: (direct and indirect: 1:2,000-1:5,000) . Immunohistochemistry: (paraffin sections (1:500-1:1,000) ) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  NAMPT

• NCBI Official Name:

  Nicotinamide phosphoribosyltransferase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

• Tested Applications:

  ELISA, IHC, WB

• Protein ID:

  NP_005737

• Physical Properties:

  Liquid