Leptin Antibody (biotin)

• Background:

  Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.

• NCBI Gene ID:

  25608

• Swiss Prot:

  P50596

• Accession Number:

  1709436

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Recombinant rat leptin.

• Clonality:

  Polyclonal

• Conjugation:

  Biotin

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.

• Applications Notes:

  ELISA: (direct and indirect: 1:500-1:5000) . Western Blot: (1:500-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  Lep

• NCBI Official Name:

  Leptin

• NCBI Organism:

  Rattus norvegicus

• Other Product Names:

  OB Gene Product; Obese Factor

• Tested Applications:

  ELISA, WB

• Protein ID:

  P50596

• Physical Properties:

  Liquid