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Leptin Antibody

CAT: 0223-36-200Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0223-36-200Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
NCBI Gene ID
25608
Swiss Prot
P50596
Accession Number
1709436
Host
Rabbit
Reactivity
Rat
Immunogen
Recombinant rat leptin.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity
Concentration
1 mg/ml
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 6 months after receipt when stored at -20˚ C.
Specificity
Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
Applications Notes
ELISA: (direct and indirect: 1:5,000-1:10,000) . Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.
Symbol
Lep
NCBI Official Name
Leptin
NCBI Organism
Rattus norvegicus
Other Product Names
OB Gene Product; Obese Factor
Tested Applications
ELISA, WB
Protein ID
P50596
Physical Properties
Liquid

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