Products for Research Use Only
RegisterLogin

HDAC4 Antibody

CAT: 0223-55-589Size: 400 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0223-55-589Size:400 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.
NCBI Gene ID
9759
Swiss Prot
P56524
Accession Number
P56524
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1052-1084 amino acids from the C-terminal region of human HDAC4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience, Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
119 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
HDAC4
NCBI Official Name
Histone deacetylase 4
NCBI Organism
Homo sapiens
Background Reference 01
Chan, J.K., et al., J. Biol. Chem. 278 (26) :23515-23521 (2003) .
Background Reference 02
Kao, G.D., et al., J. Cell Biol. 160 (7) :1017-1027 (2003) .
Background Reference 03
Zhang, C.L., et al., Mol. Cell. Biol. 22 (20) :7302-7312 (2002) .
Background Reference 04
Kirsh, O., et al., EMBO J. 21 (11) :2682-2691 (2002) .
Other Product Names
Histone deacetylase 4, HD4, HDAC4, KIAA0288
Tested Applications
WB
Protein ID
259016348
Physical Properties
Liquid

Related Products

CatalogName
79-424HDAC4 / HDAC5 / HDAC9 (Ab 246 / 259 / 220) Antibody
79-244HDAC4 / HDAC5 / HDAC9 (phospho Ser246 / Ser259 / Ser220) Antibody
79-468HDAC4 (phospho Ser632) Antibody
79-557HDAC4 (Ab 632) Antibody
70-769HDAC6 Antibody
43-003Hdac1 Antibody
22-835HDAC7 Antibody
28-136HDAC8 Antibody
27-648HDAC2 Antibody
7899-002mgHDAC2 Antibody

Alternative Products

CatalogName
20R-2316HDAC4 antibody
ABD6042HDAC4 Antibody

Popular Products