HDAC2 Antibody

• Background:

  Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.

• NCBI Gene ID:

  3066

• Swiss Prot:

  Q92769

• Accession Number:

  NP_001518

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HDAC2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  55 kDa

• Applications Notes:

  HDAC2 antibody can be used for detection of HDAC2 by ELISA at 1:12500. HDAC2 antibody can be used for detection of HDAC2 by western blot at 0.2-2.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  HDAC2

• NCBI Official Name:

  Histone deacetylase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Choi, Y.B., et al., (2004) J.Biol.Chem.279 (49), 50930-50941.

• Other Product Names:

  HDAC2, HD2, RPD3, YAF1

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  293336691

• Physical Properties:

  Liquid