CRYGS Antibody

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. This gene encodes a protein initially considered to be a beta-crystallin but the encoded protein is monomeric and has greater sequence similarity to other gamma-crystallins. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.

1427

P22914

P22914

Human, Mouse

This CRYGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-162 amino acids from the C-terminal region of human CRYGS.

Unconjugated

Primary Antibodies

Neuroscience

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

21 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

CRYGS

Beta-crystallin S

Homo sapiens

Acosta-Sampson, L., et al. J. Mol. Biol. 401 (1) :134-152 (2010)

Ma, Z., et al. Biochemistry 48 (30) :7334-7341 (2009)

Chen, J., et al. Biochemistry 48 (17) :3708-3716 (2009)

Vanita, V., et al. Mol. Vis. 15, 476-481 (2009) :

Beta-crystallin S, Gamma-S-crystallin, Gamma-crystallin S, CRYGS, CRYG8

WB

4033688