CRYGD Antibody

• Background:

  Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.

• NCBI Gene ID:

  1421

• Swiss Prot:

  P07320

• Accession Number:

  P07320

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CRYGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of human CRYGD.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  21 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CRYGD

• NCBI Official Name:

  Gamma-crystallin D

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Acosta-Sampson, L., et al. J. Mol. Biol. 401 (1) :134-152 (2010)

• Background Reference 02:

  Wang, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (30) :13282-13287 (2010)

• Background Reference 03:

  Pande, A., et al. Biochemistry 49 (29) :6122-6129 (2010)

• Background Reference 04:

  Das, P., et al. Protein Sci. 19 (1) :131-140 (2010)

• Other Product Names:

  Gamma-crystallin D, Gamma-D-crystallin, Gamma-crystallin 4, CRYGD, CRYG4

• Tested Applications:

  WB

• Protein ID:

  2506321

• Physical Properties:

  Liquid