CD68 Antibody [SPM130]

• Background:

  This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

• NCBI Gene ID:

  968

• Swiss Prot:

  P34810

• Host:

  Mouse

• Reactivity:

  Human, Monkey, Rabbit, Cat

• Immunogen:

  A human alveolar macrophage subcellular fraction was used as the immunogen for this anti-CD68 antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM130

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Specificity:

  Does not react with Pig, Dog, Chicken

• Applications Notes:

  1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CD68

• NCBI Official Name:

  CD68 molecule

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Macrosialin, Gp110, CD68, CD68

• Tested Applications:

  Flow, IF, IHC

• Physical Properties:

  Liquid